A high-throughput screening system for human genes extending life-span
C. Chen and R. Contreras
Fundamental and Applied Molecular Biology, Ghent Universityand VIB, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
We developed a high-throughput functional genomic strategy that allows
identification of human genes prolonging life-span in the baker's yeast
Saccharomyces cerevisiae. The method is based on isolating yeast mother
cells with extended number of cell divisions as indicated by the
increased number of bud scars on their surface. Screening of a human
HepG2 cDNA expression library in yeast resulted in the isolation of 12
yeast transformants with a potentially prolonged life-span. The
transgene in one of the lines was identified as ferritin light chain
(FTL) and studied in more detail. Yeast cells containing FTL showed an
enhanced iron and H2O2 resistance, a reduced cell death rate and an
increased number of cell divisions. Our data demonstrate that this
method permits a fast screening of libraries for hunting genes involved
in ageing processes.
Key words:
aging, lifespan, stress, yeast
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