Observation of Preventive and Therapeutic Effects of Antithrombin-Ⅲ on Experimental Disseminated Intravascular Coagulation





Hongjiang Dong, Shuhua Zhang, Lin Li

Dept. of Technique, Tianjin Zhengzhao Bio-technique Development Co Ltd;

No.301 Jiefang South Road, Hexi District, Tianjin 300202, CHINA



Objective: To observe the preventive and therapeutic effects of antithrombin-III (AT-III) on experimental disseminated intravascular coagulation (DIC) in rabbits. Methods: Rabbits were at random divided into the control and AT-III plus lipopolysaccharide (LPS) or AT-III plus thromboplastin (Tp) groups. Experimental DIC was induced by injection of LPS or Tp. AT-III was intravenously injected after the injection of LPS or Tp. 2.5 hours later, blood sample were drawn and the concentration of fibrinogen, prothrombin time (PT), 3P test, platelet count, AST and CK was measured. Results: As compared with the controls, it was found that the platelet count and plasma concentration of fibrinogen were significantly decreased and the PT time showed no significant difference, but the positive degree of 3P test was remarkably decreased and the pathologic changes of the heart, liver, lung and kindney were slight in the high-dose AT-III group. Conclusion: AT-III can inhibit the DIC and inflammation response caused by LPS or Tp.




Key words: Antithrombin-III; Lipopolysaccharide; Disseminated intravascular coagulation; Throm-boplastin; Rabbit







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