Aging Research Enabled by Patient-Specific Embryonic Stem Cells Derived from Human SCNT-Blastocysts
Woo Suk Hwang, Sung Il Roh, Byeong Chun Lee, Sung Keun Kang, Dae Kee Kwon, Sue Kim, Sun Jong Kim, Sun Woo Park, Hee Sun Kwon, Chang Kyu Lee, Jung Bok Lee, Jin Mee Kim, Curie Ahn, Sun Ha Paek, Sang Sik Chang, Jung Jin Koo, Hyun Soo Yoon, Jung Hye Hwang, Youn Young Hwang, Ye Soo Park, Sun Kyung Oh, Hee Sun Kim, Jong Hyuk Park, Shin Yong Moon, Gerald Schatten
Pittsburgh Development Center, Magee-Womens Research Institute, Departments of Obstetrics-Gynecology-Reproductive Sciences and Cell Biology-Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Aging research and the biology of sensence may benefit from the
efficient derivations of human embryonic stem cells derived from cloned
blastocysts. Patient-specific, immune-matched human embryonic stem
cells (hESC) are anticipated to be of great biomedical importance for
studies of disease and development, and to advance clinical
deliberations for stem cell transplantation. Eleven hESC lines were
established by nuclear transfer (SCNT; NT) of skin cells from patients
with disease or injury into donated oocytes. These lines (NT-hESCs),
grown on human feeders from the same NT-donor or genetically-unrelated
individuals, were established at high rates, regardless of NT-donor sex
or age. NT-hESCs were pluripotent, chromosomally normal, and match
NT-patient's DNA. Major Histocompatibility Complex (MHC) identity of
each NT-hESC with the patient's showed immunological compatibility,
important for eventual transplantation. With the generation of these
NT-hESCs, evaluations of genetic and epigenetic stability can be made.
Additional work remains regarding the development of reliable directed
differentiation and the elimination of remaining animal components.
Prior to clinical use of these cells, preclinical evidence is required
to prove that transplantation of differentiated-NT-hESCs can be safe,
effective and tolerated.
Key words:
Human Embryonic Stem Cells; Nuclear Transfer; Aging
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